Product Name :
Rabbit anti-PAFR Polyclonal Antibody
Synonym :
Platelet-activating factor receptor; PAF receptor; Ptafr; OTTHUMP00000004041; OTTMUSP00000010350; PAF R; PAF-R; PAFR; Platelet activating factor receptor; PTAFR; PTAFR_HUMAN; RP23-470B20.1
Host :
Rabbit
Species Reactivity:
Human, Mouse, Rat
Specificity :
PAFR/PAF Receptor
Predicted Reactivity:
Chicken, Dog, Cow, Rabbit
Applications :
WB=1:500-2000, IHC-P=1:100-500
Immunogen:
KLH conjugated synthetic peptide derived from human PAFR:231-342/342
Concentration :
1mg/ml
Purification :
affinity purified by Protein A
Clonality:
Polyclonal Antibody
Storage Temp.:
Store at -20 ° C for one yearAvoid repeated freeze/that cycles
Research areas :
Cardiovascular Cell biology Immunology Neurobiology Signal transduction Cell membrane receptors
Background :
The PAF receptor binds platelet-activating factor (PAF) and is thought to mediate its action via a G protein that activates a phosphatidylinositol-calcium second messenger system. PAF is a chemotactic phospholipid mediator that possesses potent inflammatory, smooth-muscle contractile and hypotensive activity. It has been implicated as a mediator in diverse pathologic processes, such as allergy, asthma, septic shock, arterial thrombosis, and inflammatory processes. The PAF receptor is induced by granulocyte macrophage colony-stimulating factor (GM-CSF), interleukin-5 and n-butyrate. A diverse range of compounds act as PAF receptor antagonists; these may be useful pharmacologically.
UniProt :
P25105
Additional information:
Product Details FAQ Citations(0) Video Pictures Documents |Overview |Synonym Platelet-activating factor receptor; PAF receptor; Ptafr; OTTHUMP00000004041; OTTMUSP00000010350; PAF R; PAF-R; PAFR; Platelet activating factor receptor; PTAFR; PTAFR_HUMAN; RP23-470B20.1 |Host Rabbit |Specificity PAFR/PAF Receptor |Species Reactivity Human, Mouse, Rat |Predicted Reactivity Chicken, Dog, Cow, Rabbit |Applications WB=1:500-2000, IHC-P=1:100-500 |Immunogen KLH conjugated synthetic peptide derived from human PAFR:231-342/342 |Properties |Concentration 1mg/ml |Purification affinity purified by Protein A |Clonality Polyclonal Antibody |Isotype IgG |Storage Temp. Store at -20 ° C for one yearAvoid repeated freeze/that cycles |Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |Research areas Cardiovascular Cell biology Immunology Neurobiology Signal transduction Cell membrane receptors |Target |Background The PAF receptor binds platelet-activating factor (PAF) and is thought to mediate its action via a G protein that activates a phosphatidylinositol-calcium second messenger system. PAF is a chemotactic phospholipid mediator that possesses potent inflammatory, smooth-muscle contractile and hypotensive activity. It has been implicated as a mediator in diverse pathologic processes, such as allergy, asthma, septic shock, arterial thrombosis, and inflammatory processes. The PAF receptor is induced by granulocyte macrophage colony-stimulating factor (GM-CSF), interleukin-5 and n-butyrate. A diverse range of compounds act as PAF receptor antagonists; these may be useful pharmacologically. |Cellular localization Cell membrane; |UniProt P25105 | Sample: Lane1: Lung(Rat) Lysate at 30 ugLane2: Brain(Rat) Lysate at 30 ugPrimary: Anti-PTAFR (abs122956) at 1:200 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG at 1: 3000 dilution; Predicted band size : 38kDObserved band size : 38kD| Sample: Lymph (Mouse) Lysate at 30 ugPrimary: Anti- RAFR/PAF (abs122956) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilutionPredicted band size: 38 kDObserved band size: 38 kD| Blank control: 293T cells(blue). Primary Antibody:Rabbit Anti-PAFR/PAF antibody, Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.ProtocolThe cells were fixed with 2% paraformaldehyde (10 min). Primary antibody were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed. |Tips:This product is for research use only. Not for use in diagnostic prodcedures.
Rabbit anti-PAFR Polyclonal Antibody
Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.Related websites: https://www.medchemexpress.com/antibodies.html
2568868-35-7 Biological Activity 875320-31-3 Formula PMID:29083747 MedChemExpress (MCE) offers a wide range of high-quality research chemicals and biochemicals (novel life-science reagents, reference compounds and natural compounds) for scientific use. We have professionally experienced and friendly staff to meet your needs. We are a competent and trustworthy partner for your research and scientific projects.Related websites: https://www.medchemexpress.com
